To explore the relationship between the behavior of critical membrane sites and cell function by observing the transient state of membranes composed of dynamic biomolecules.
Masaki Tsuchiya
School of Pharmaceutical Sciences, University of Shizuoka
Yuji Hara
School of Pharmaceutical Sciences, University of Shizuoka
Selected publications
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Masaki Tsuchiya, Nobuhiko Tachibana, Itaru Hamachi “Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling” STAR Protocols, 2023
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Masaki Tsuchiya, Nobuhiko Tachibana, Kohjiro Nagao, Tomonori Tamura, Itaru Hamachi “Organelle-selective click labeling coupled with flow cytometry allows high-throughput CRISPR screening of genes involved in phosphatidylcholine metabolism” Cell Metabolism, 2023
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Kotaro Hirano, Masaki Tsuchiya, Seiji Takabayashi, Kohjiro Nagao, Yasuo Kitajima, Yusuke Ono, Keiko Nonomura, Yasuo Mori, Masato Umeda, Yuji Hara “The mechanosensitive Ca2+-permeable ion channel PIEZO1 promotes satellite cell function in skeletal muscle regeneration” Life Science Alliance, 6, e202201783, 2022.
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Masaki Tsuchiya, Yuji Hara, Masaki Okuda, Karin Itoh, Ryotaro Nishioka, Akifumi Shiomi, Kohjiro Nagao, Masayuki Mori, Yasuo Mori, Junichi Ikenouchi, Ryo Suzuki, Motomu Tanaka, Tomohiko Ohwada, Junken Aoki, Motoi Kanagawa, Tatsushi Toda, Yosuke Nagata, Ryoichi Matsuda, Yasunori Takayama, Makoto Tominaga, Masato Umeda “Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation” Nature Communications, 9, 2049, 2018.
Group A02 (Tsuchiya and Hara)
Development of critical membrane site imaging technology for visualizing local changes in membrane lipids